| Gene | Slitrk2 |
| Chromosome | X |
| Deletion Start | 66,653,888 |
| Deletion End | 66,656,446 |
| Deletion Size | 2,559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,654,816 | 66,654,836 | GCCACCCAAAATGAGAAATCG |
| TUP | 66,654,849 | 66,654,866 | AGTGACTGTGTCAAAAGA |
| TUR | 66,654,875 | 66,654,893 | ACCATGATCGGGCCAAAGC |
| TDF | 66,655,535 | 66,655,555 | GGACCGAACATGCCAATTCTC |
| TDP | 66,655,566 | 66,655,585 | CAATGAGGTGACTTGTGAGT |
| TDR | 66,655,591 | 66,655,611 | TATCTCCCCTGCATGCTTAGC |
PCR Primers
| Primer | Sequence |
| SU | AACTGCTTTGAAATACGCAATG |
| SD | GGGAACGGGATTAGGAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 317 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10001A-B8 | VGB6 | C57BL/6NTac | |
| 10001A-B11 | VGB6 | C57BL/6NTac | |
| 10001A-F11 | VGB6 | C57BL/6NTac | |
| 10001A-H10 | VGB6 | C57BL/6NTac | |
