| Gene | Pvrig-ps |
| Chromosome | 5 |
| Deletion Start | 138,341,991 |
| Deletion End | 138,344,506 |
| Deletion Size | 2,516 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,342,368 | 138,342,389 | CCAAACAGCGTCTCTGTCACTC |
| TUP | 138,342,393 | 138,342,420 | CCATGGGACAATCCAAAGCAAGAAGCTC |
| TUR | 138,342,425 | 138,342,444 | CAGCAGAATGTGGTGTTGGC |
| TDF | 138,342,951 | 138,342,969 | AGACCATCCCTGGCCACTG |
| TDP | 138,342,973 | 138,342,998 | TGGATCCACCATCCCAATACCTTCCC |
| TDR | 138,343,003 | 138,343,022 | GCTAAACAGGGTGGCTGGAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCACTACTACTGCAGATC |
| SD | AGTTGTCAGCTGGTAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 331 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10003A-C6 | VGB6 | C57BL/6NTac | |
| 10003A-C7 | VGB6 | C57BL/6NTac | |
| 10003A-D11 | VGB6 | C57BL/6NTac | |
| 10003A-E7 | VGB6 | C57BL/6NTac | |
