| Gene | Slitrk1 |
| Chromosome | 14 |
| Deletion Start | 108,913,273 |
| Deletion End | 108,911,161 |
| Deletion Size | 2,113 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,912,715 | 108,912,733 | TCGACCTCCGTGGAAACAG |
| TUP | 108,912,694 | 108,912,712 | TGAAAACACTGCCCTATGA |
| TUR | 108,912,670 | 108,912,689 | CCAGGGATCTGCTCCAAGAC |
| TDF | 108,911,377 | 108,911,395 | TCGTCACCTCCGCCTTCAC |
| TDP | 108,911,349 | 108,911,366 | ATGCTCGTGTTTATCCTG |
| TDR | 108,911,323 | 108,911,342 | CTTCTCTTGGACCGCTTTCG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCCTGGATAGTTTGTGG |
| SD | GCTCAGAGCAAAGAGCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 316 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10005C-A6 | VGB6 | C57BL/6NTac | |
| 10005C-B1 | VGB6 | C57BL/6NTac | |
| 10005C-F6 | VGB6 | C57BL/6NTac | |
| 10005C-G3 | VGB6 | C57BL/6NTac | |
