Gene | Zzef1 |
Chromosome | 11 |
Deletion Start | 72,796,279 |
Deletion End | 72,831,740 |
Deletion Size | 35,462 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 72,796,703 | 72,796,724 | TCGCCAATAAGGTCCTGAAGAG |
TUP | 72,796,734 | 72,796,753 | TGGCTGGCCTCCGCAAAGCA |
TUR | 72,796,759 | 72,796,778 | AGAACCCGAGGACCCTATGG |
TDF | 72,831,638 | 72,831,661 | CTGACCACTTGGACACACAGTATG |
TDP | 72,831,663 | 72,831,687 | CGCCTCATCCCTCATCTCATCCATG |
TDR | 72,831,689 | 72,831,709 | GCAGGTTCTGCCTGACAGATG |
PCR Primers
Primer | Sequence |
SU | GACGGATACGTTAACGGGTG |
SD | CTCCAGCCAAGTCTCTCTAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 353 bp | - | + | + |
TUF | TUR | 76 bp | + | + | - |
TDF | TDR | 72 bp | + | + | - |
NeoFwd | SD | 322 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
10007B-A4 | VGB6 | C57BL/6NTac | |