10007   Zzef1     Status: Germline transmission achieved

GeneZzef1
Chromosome11
Deletion Start72,796,279
Deletion End72,831,740
Deletion Size35,462
Genome Build38
MGI IDMGI:2444286
NCBI Gene ID195018
BAC ID151L18
BAC LibraryRPCI 23
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF72,796,70372,796,724TCGCCAATAAGGTCCTGAAGAG
TUP72,796,73472,796,753TGGCTGGCCTCCGCAAAGCA
TUR72,796,75972,796,778AGAACCCGAGGACCCTATGG
TDF72,831,63872,831,661CTGACCACTTGGACACACAGTATG
TDP72,831,66372,831,687CGCCTCATCCCTCATCTCATCCATG
TDR72,831,68972,831,709GCAGGTTCTGCCTGACAGATG

PCR Primers

PrimerSequence
SUGACGGATACGTTAACGGGTG
SDCTCCAGCCAAGTCTCTCTAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev353 bp-++
TUFTUR76 bp++-
TDFTDR72 bp++-
NeoFwdSD322 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10007B-A4VGB6C57BL/6NTac