| Gene | Lgi2 |
| Chromosome | 5 |
| Deletion Start | 52,566,102 |
| Deletion End | 52,537,943 |
| Deletion Size | 28,160 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,565,695 | 52,565,714 | ACGGGAATCCAGACAGGTCC |
| TUP | 52,565,669 | 52,565,693 | CGTGACAGTTTCTCATTGTGGCCCG |
| TUR | 52,565,648 | 52,565,667 | TAGGTCTGATGCAGGTGGCC |
| TDF | 52,556,600 | 52,556,619 | ACTGGGTCCGTCTCAGAGGC |
| TDP | 52,556,569 | 52,556,598 | CAAATGATCACATACACTTGGGTGGACCAC |
| TDR | 52,556,538 | 52,556,558 | GGTGCACAATCCTCTTCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TTCATTGGTCCGGGCCCG |
| SD | GATGTCGCTTGTAAACAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 421 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10011B-B10 | VGB6 | C57BL/6NTac | |
| 10011B-E10 | VGB6 | C57BL/6NTac | |
| 10011B-F4 | VGB6 | C57BL/6NTac | |
| 10011B-G3 | VGB6 | C57BL/6NTac | |
| 10011C-D7 | VGB6 | C57BL/6NTac | |
