| Gene | Nlgn3 |
| Chromosome | X |
| Deletion Start | 101,301,969 |
| Deletion End | 101,320,262 |
| Deletion Size | 18,294 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,302,264 | 101,302,283 | GGAACGCCACACACTTTCCC |
| TUP | 101,302,285 | 101,302,308 | CAGTGTGCCCCCAGAACATCCACA |
| TUR | 101,302,312 | 101,302,331 | CAGCATGACTTCGGGCACAG |
| TDF | 101,310,603 | 101,310,623 | TTGCTTTGCCAATTCCAGTCC |
| TDP | 101,310,625 | 101,310,648 | CGAGGCCCTTGTGCTGCATTTGCT |
| TDR | 101,310,652 | 101,310,672 | TCTGTGCATATAACCCTGCCC |
PCR Primers
| Primer | Sequence |
| SU | TCTTTCCACAGGCCTGTCTG |
| SD | CTCCCGAGTCTGCAGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 451 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10013C-A4 | VGB6 | C57BL/6NTac | |
| 10013C-B11 | VGB6 | C57BL/6NTac | |
| 10013C-E5 | VGB6 | C57BL/6NTac | |
| 10013C-E10 | VGB6 | C57BL/6NTac | |
| 10013D-B12 | VGB6 | C57BL/6NTac | |
| 10013D-H7 | VGB6 | C57BL/6NTac | |
