| Gene | Glp2r |
| Chromosome | 11 |
| Deletion Start | 67,764,824 |
| Deletion End | 67,728,255 |
| Deletion Size | 36,570 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,764,401 | 67,764,421 | CCTGACCAGCAGCCATATTCC |
| TUP | 67,764,369 | 67,764,395 | TGAACAGGCCTTTCAGTCCCATGTCAT |
| TUR | 67,764,341 | 67,764,360 | CTGTCCCATGCAGCTGAATG |
| TDF | 67,728,796 | 67,728,818 | TGTGTGAAATGGGAGTGTTGTGA |
| TDP | 67,728,763 | 67,728,791 | CCCATCTCCTATGAATTGTGGCAACTGAA |
| TDR | 67,728,741 | 67,728,761 | TGGCTGCCCATAGTCAATGTC |
PCR Primers
| Primer | Sequence |
| SU | CCAAGTGTGAGCAGTATTTCC |
| SD | AACTAACGACCCAAGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 551 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10016C-F2 | VGB6 | C57BL/6NTac | |
