| Gene | Cst8 |
| Chromosome | 2 |
| Deletion Start | 148,799,969 |
| Deletion End | 148,805,522 |
| Deletion Size | 5,554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,800,006 | 148,800,028 | TCCTTGATCCTCTTCATCATTCC |
| TUP | 148,800,031 | 148,800,053 | ATCCACACCAACTGCCAGGGCCA |
| TUR | 148,800,059 | 148,800,081 | TCTGTGCCTTCACTTCATTCTTG |
| TDF | 148,805,381 | 148,805,401 | CATGCTGCTGATGCTCTTTGC |
| TDP | 148,805,426 | 148,805,452 | TGCAGCTTTCTGGTAGGAGCACTTCCC |
| TDR | 148,805,454 | 148,805,474 | ACAGGTTGAACTCGCCATTCC |
PCR Primers
| Primer | Sequence |
| SU | CTTTTGTATGACGATGTAGGG |
| SD | TCTCACTTTCTCATTCTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 445 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10017A-A9 | VGB6 | C57BL/6NTac | |
| 10017A-B3 | VGB6 | C57BL/6NTac | |
| 10017A-G10 | VGB6 | C57BL/6NTac | |
| 10017B-H4 | VGB6 | C57BL/6NTac | |
| 10017B-G5 | VGB6 | C57BL/6NTac | |
| 10017B-B9 | VGB6 | C57BL/6NTac | |
