| Gene | Gdf2 |
| Chromosome | 14 |
| Deletion Start | 33,941,136 |
| Deletion End | 33,945,641 |
| Deletion Size | 4,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,942,373 | 33,942,394 | GGTTTGGCATCTTGCTCTGAAG |
| TUP | 33,942,396 | 33,942,420 | ACCAGGAGCCCAAGGCCTCTCTCTT |
| TUR | 33,942,438 | 33,942,457 | CTGGAACCCTTGGGCATTTG |
| TDF | 33,945,448 | 33,945,468 | CAGACCCTGGTGCATCTCAAG |
| TDP | 33,945,474 | 33,945,497 | CAGCAGGCTTTGCCCACCTTTGTG |
| TDR | 33,945,506 | 33,945,526 | TGGAGATGGGACTCAGTTTGG |
PCR Primers
| Primer | Sequence |
| SU | CAGTAGTCAGCATCCTTTCC |
| SD | AGTTTCTGCCTGGTTTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10018G-C5 | VGB6 | C57BL/6NTac | |
