| Gene | Ch25h |
| Chromosome | 19 |
| Deletion Start | 34,475,123 |
| Deletion End | 34,474,154 |
| Deletion Size | 970 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,474,750 | 34,474,770 | TGAGCTCGTCCAGCTCCTAAG |
| TUP | 34,474,722 | 34,474,746 | CGTCCTGATCTGCCTGCTGCTCTTC |
| TUR | 34,474,697 | 34,474,715 | GGTGCCACGCGAAGATCTC |
| TDF | 34,474,605 | 34,474,623 | GCTGGCGACCCAATACATG |
| TDP | 34,474,578 | 34,474,602 | CTTCTGGGAGCTGCTTTCGCTGACC |
| TDR | 34,474,547 | 34,474,565 | GAAGCACCGCGACGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTCATTAGTACATCAGCTG |
| SD | GTCACTGCCCAGCAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 389 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 372 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10020A-A4 | VGB6 | C57BL/6NTac | |
| 10020A-A7 | VGB6 | C57BL/6NTac | |
| 10020A-A9 | VGB6 | C57BL/6NTac | |
| 10020A-A11 | VGB6 | C57BL/6NTac | |
| 10020A-B2 | VGB6 | C57BL/6NTac | |
| 10020A-B5 | VGB6 | C57BL/6NTac | |
| 10020K-A5 | VGB6 | C57BL/6NTac | |
