| Gene | Fgf11 |
| Chromosome | 11 |
| Deletion Start | 69,801,622 |
| Deletion End | 69,798,178 |
| Deletion Size | 3,445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,800,674 | 69,800,694 | CCCACCAGGAGAGGAGAGATG |
| TUP | 69,800,647 | 69,800,671 | TGGACGGGAATCACATACCAGCCCT |
| TUR | 69,800,619 | 69,800,639 | GCCAGAGGGACTCCCTAACTG |
| TDF | 69,798,622 | 69,798,640 | GGAAGGCCGGGTCATGAAG |
| TDP | 69,798,591 | 69,798,618 | CAGCTGCCTTGGTCTTCTTGACTCGGTT |
| TDR | 69,798,569 | 69,798,588 | AGGAGCTTGGGCACAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTACAGAGGCCAGAGAG |
| SD | GGATCTAAGAGCTCAGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 409 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 282 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10021A-C12 | VGB6 | C57BL/6NTac | |
| 10021A-H7 | VGB6 | C57BL/6NTac | |
