| Gene | Gpr34 |
| Chromosome | X |
| Deletion Start | 13,639,216 |
| Deletion End | 13,640,391 |
| Deletion Size | 1,176 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,639,497 | 13,639,516 | TGTGGCTGTTGCAGACCTTC |
| TUP | 13,639,519 | 13,639,536 | CTCATCTTCTGCCTCCCT |
| TUR | 13,639,539 | 13,639,562 | TGGTTGATGTGATACATTATGCGG |
| TDF | 13,640,250 | 13,640,272 | TCAAAGTGAAGCAAGCAGAAGTG |
| TDP | 13,640,274 | 13,640,291 | AAGCACTTCAGAATTTAA |
| TDR | 13,640,293 | 13,640,312 | TCATGCAAGGAATGTCCTGG |
PCR Primers
| Primer | Sequence |
| SU | TGAGCATGCCACAGCAACAG |
| SD | GATTTTGAAGTAATACTCATGTTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 405 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | 465 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10023A-D1 | VGB6 | C57BL/6NTac | |
| 10023A-D4 | VGB6 | C57BL/6NTac | |
| 10023B-A5 | VGB6 | C57BL/6NTac | |
| 10023B-D9 | VGB6 | C57BL/6NTac | |
| 10023B-F4 | VGB6 | C57BL/6NTac | |
| 10023C-D10 | VGB6 | C57BL/6NTac | |
