| Gene | Chst7 |
| Chromosome | X |
| Deletion Start | 20,059,699 |
| Deletion End | 20,061,173 |
| Deletion Size | 1,475 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,060,050 | 20,060,069 | CGTCCTTCTTGGGCGAACTC |
| TUP | 20,060,072 | 20,060,098 | CAACCAGCACCCGGACGTTTTCTACTT |
| TUR | 20,060,105 | 20,060,123 | CCACAGATGCCACATGGGC |
| TDF | 20,060,876 | 20,060,894 | GCTGCGCTTCTCTGGGTTG |
| TDP | 20,060,898 | 20,060,920 | ACACTCGCCGCGCTTGATGCCTT |
| TDR | 20,060,922 | 20,060,940 | CCCGCGTCATATTGAATGC |
PCR Primers
| Primer | Sequence |
| SU | GAGAAGTTTCGGGTACCATGG |
| SD | CTTCCAAGACCTCCTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 330 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10024B-B4 | VGB6 | C57BL/6NTac | |
| 10024B-C8 | VGB6 | C57BL/6NTac | |
| 10024B-C9 | VGB6 | C57BL/6NTac | |
| 10024B-E4 | VGB6 | C57BL/6NTac | |
| 10024B-F5 | VGB6 | C57BL/6NTac | |
| 10024B-H4 | VGB6 | C57BL/6NTac | |
| 10024C-E5 | VGB6 | C57BL/6NTac | |
