| Gene | S100a14 |
| Chromosome | 3 |
| Deletion Start | 90,527,269 |
| Deletion End | 90,528,218 |
| Deletion Size | 950 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,527,271 | 90,527,289 | AGTGTCGGTCAGCCAATGC |
| TUP | 90,527,299 | 90,527,324 | CCCGTTCATCTGGCCTGTCTTTCTGT |
| TUR | 90,527,344 | 90,527,362 | GGGCGCAAACCCTCATAAC |
| TDF | 90,527,550 | 90,527,569 | GAGGCCACTGTGCCCTAGAC |
| TDP | 90,527,576 | 90,527,601 | AATCCTGCCCACTCCCTCTCCTCTGG |
| TDR | 90,527,616 | 90,527,641 | AACAGGGTAGAAAGTGGCTAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | GATCAGTGAAGGTGAAGGCA |
| SD | CCTTGAGATGAGGACTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 329 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 290 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10025A-B3 | VGB6 | C57BL/6NTac | |
| 10025A-F2 | VGB6 | C57BL/6NTac | |
| 10025A-G8 | VGB6 | C57BL/6NTac | |
| 10025A-A1 | VGB6 | C57BL/6NTac | |
