| Gene | Ppbp |
| Chromosome | 5 |
| Deletion Start | 90,768,587 |
| Deletion End | 90,769,412 |
| Deletion Size | 826 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,769,248 | 90,769,267 | CCTCCCTCCTCATGGATCTG |
| TUP | 90,769,271 | 90,769,301 | TGATGGTCTGTGTACAACAGCCTCTCTGTCA |
| TUR | 90,769,306 | 90,769,326 | CAGTGTGGCTCTGCCAAAGAG |
| TDF | 90,768,756 | 90,768,775 | GGCTGCTTCCCTTCATCATG |
| TDP | 90,768,778 | 90,768,802 | CCATGAGCACCACAAGCCGCTTAGA |
| TDR | 90,768,805 | 90,768,823 | ATGCACATACCCCAAGCCC |
PCR Primers
| Primer | Sequence |
| SU | ACTGATGCAGAACTGGTAGC |
| SD | TGCTTAGCCAAGAACTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10026B-A1 | VGB6 | C57BL/6NTac | |
| 10026B-A2 | VGB6 | C57BL/6NTac | |
| 10026B-B5 | VGB6 | C57BL/6NTac | |
| 10026B-C11 | VGB6 | C57BL/6NTac | |
