| Gene | Wnt6 |
| Chromosome | 1 |
| Deletion Start | 74,772,130 |
| Deletion End | 74,784,701 |
| Deletion Size | 12,572 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,772,710 | 74,772,729 | TGCCTACCACCTCACCATTG |
| TUP | 74,772,731 | 74,772,755 | TGGCCCCTTCAGCATTGGCAGTAGT |
| TUR | 74,772,759 | 74,772,779 | CCCATTTGCATTCCCTATTCC |
| TDF | 74,783,879 | 74,783,900 | ACCCTGCGATCTCTGCTCTATC |
| TDP | 74,783,907 | 74,783,934 | TCCCTCTCTCACTCCTCCATCCTTGTCA |
| TDR | 74,783,943 | 74,783,967 | TCAAGAAAAGAACCACTCAGAAAGG |
PCR Primers
| Primer | Sequence |
| SU | TCTGATTTCTTCTCTCCAGCG |
| SD | AGGTGGAATGATAGACTCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 419 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10028A-D5 | VGB6 | C57BL/6NTac | |
| 10028A-E7 | VGB6 | C57BL/6NTac | |
| 10028A-E10 | VGB6 | C57BL/6NTac | |
| 10028A-E12 | VGB6 | C57BL/6NTac | |
| 10028A-H8 | VGB6 | C57BL/6NTac | |
| 10028A-H5 | VGB6 | C57BL/6NTac | |
