| Gene | Igfbp4 |
| Chromosome | 11 |
| Deletion Start | 99,041,481 |
| Deletion End | 99,051,579 |
| Deletion Size | 10,099 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,041,886 | 99,041,906 | ACCCTCCTCCCTGCAACTTAG |
| TUP | 99,041,908 | 99,041,930 | TCCCATTCTGGCTGGCCTGGAAG |
| TUR | 99,041,958 | 99,041,977 | TGCCACGTACCTCCCTTCTC |
| TDF | 99,051,125 | 99,051,147 | GCCTCTGCTGTCAACTGTAATCC |
| TDP | 99,051,149 | 99,051,162 | ATGCTTCAAGTCCC |
| TDR | 99,051,171 | 99,051,193 | TGGAGATGTTTCAGCTTGTTGTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCGGGCAGCCGCTCAGC |
| SD | TCAGACCTCTTGGGGGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 328 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10029A-F7 | VGB6 | C57BL/6NTac | |
| 10029B-F2 | VGB6 | C57BL/6NTac | |
