| Gene | Ghrhr |
| Chromosome | 6 |
| Deletion Start | 55,376,369 |
| Deletion End | 55,388,300 |
| Deletion Size | 11,932 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,376,402 | 55,376,422 | GGCTACCCGTATCCTCTGCTT |
| TUP | 55,376,425 | 55,376,452 | TGGCTACTTACAACTCCGCACAGGCTCA |
| TUR | 55,376,460 | 55,376,480 | CACCACCCAAGAGAACAAAGG |
| TDF | 55,388,122 | 55,388,141 | GGAGTGGCAGTGGGATCTTC |
| TDP | 55,388,146 | 55,388,172 | CATCCTCTCCCTTGTCTGTGGAGGTGA |
| TDR | 55,388,188 | 55,388,206 | TCGTGGCCATACCATTTGC |
PCR Primers
| Primer | Sequence |
| SU | GTGGTGTAGCAGAGCTCAG |
| SD | ATGGCTGCTGACAAGAGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 340 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 365 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10030C-C11 | VGB6 | C57BL/6NTac | |
| 10030C-F5 | VGB6 | C57BL/6NTac | |
