| Gene | Nmur1 |
| Chromosome | 1 |
| Deletion Start | 86,388,138 |
| Deletion End | 86,386,296 |
| Deletion Size | 1,843 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,387,985 | 86,388,004 | GATCTTCGTGGTGGGCACTC |
| TUP | 86,387,958 | 86,387,979 | AACGGGCTGACCTGCACCGTCA |
| TUR | 86,387,937 | 86,387,956 | GCATAGTCTTGTTGCGCAGG |
| TDF | 86,386,496 | 86,386,515 | AGACCTTCCTGCAAGCCCTG |
| TDP | 86,386,468 | 86,386,490 | TGGAACCCAGTGCTGTCATCGCC |
| TDR | 86,386,447 | 86,386,466 | GGGAGCCATGATAGGGTTGG |
PCR Primers
| Primer | Sequence |
| SU | GCACTCCGGATACTCTGTAG |
| SD | CATGTGGTAGATGCCGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 341 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 351 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10032A-C3 | VGB6 | C57BL/6NTac | |
| 10032A-G6 | VGB6 | C57BL/6NTac | |
| 10032B-A9 | VGB6 | C57BL/6NTac | |
