| Gene | Slitrk5 |
| Chromosome | 14 |
| Deletion Start | 111,678,930 |
| Deletion End | 111,681,830 |
| Deletion Size | 2,901 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,679,686 | 111,679,706 | GGATTGGTTGGACAGCATCTC |
| TUP | 111,679,715 | 111,679,738 | CACAGACCACATCGCCCACAAGGG |
| TUR | 111,679,751 | 111,679,771 | CCAAATCCCTGCCGTGTAGAC |
| TDF | 111,680,917 | 111,680,937 | GCATCCTCTGTGCCCTTATCC |
| TDP | 111,680,952 | 111,680,977 | TCTGCTGCTGGTTTTCATCATGTCCG |
| TDR | 111,680,979 | 111,680,997 | AAGGCCTGCTGCCACAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCGTTTGGTGGCATGCTC |
| SD | GACCACAACCTGTTTCAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 368 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10033C-H2 | VGB6 | C57BL/6NTac | |
