| Gene | Wnt2b |
| Chromosome | 3 |
| Deletion Start | 104,961,469 |
| Deletion End | 104,946,946 |
| Deletion Size | 14,524 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,961,216 | 104,961,236 | CTGCTTAGGCACCAGAATTGC |
| TUP | 104,961,184 | 104,961,213 | TGGGACTGTGTCCTTCCTCTAATTCCTCCT |
| TUR | 104,961,161 | 104,961,180 | CCTCACTGGCCACTGGAAAC |
| TDF | 104,947,190 | 104,947,211 | CCCTGACTCAGGTTCCCTAGGT |
| TDP | 104,947,145 | 104,947,174 | CCCATCTGTTCCTTTAGAAGTCTTGCTGCA |
| TDR | 104,947,122 | 104,947,142 | CGGCCACAACACATGATTTCA |
PCR Primers
| Primer | Sequence |
| SU | CTCAGCGCTCGTCGGTCTC |
| SD | CCATCCCCTATCAACACGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 317 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10034B-D4 | VGB6 | C57BL/6NTac | |
