| Gene | Cfi |
| Chromosome | 3 |
| Deletion Start | 129,848,549 |
| Deletion End | 129,875,140 |
| Deletion Size | 26,592 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,848,824 | 129,848,844 | GCGCAGCTGAAGGTAGGAAAG |
| TUP | 129,848,848 | 129,848,871 | TGCGTAAGCAAGCCATTCGGACAA |
| TUR | 129,848,879 | 129,848,898 | CCGAAAGGATGGCCTATCAC |
| TDF | 129,874,935 | 129,874,954 | TCCATTGATGCCTGCAAAGG |
| TDP | 129,874,961 | 129,874,986 | TATCCTCACAGACCAAGGGGCCTCCG |
| TDR | 129,874,999 | 129,875,019 | TCACAATGCCCCAAACGTAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTACCAGCCTCACTGTG |
| SD | ACCACCTTGTATGCTTAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 435 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 280 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10035B-A12 | VGB6 | C57BL/6NTac | |
