| Gene | Cxcl16 |
| Chromosome | 11 |
| Deletion Start | 70,459,444 |
| Deletion End | 70,455,383 |
| Deletion Size | 4,062 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,458,978 | 70,458,998 | TGGGACCATCGATCTGAACAC |
| TUP | 70,458,951 | 70,458,976 | CAGTCCAAGTCCCGGAGCCTAATTCC |
| TUR | 70,458,909 | 70,458,929 | CCCCACTACAGCTGGAAATCC |
| TDF | 70,455,747 | 70,455,767 | TAGCACACCAGCTTGGGTACC |
| TDP | 70,455,721 | 70,455,744 | TGCTGTCCCTCCTGGCCATTGTCT |
| TDR | 70,455,700 | 70,455,719 | CCATGGCTGCAGTGAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGGAGCTGGGCGCAAAGC |
| SD | AGCTAAACAACAAAACCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 347 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10037B-A9 | VGB6 | C57BL/6NTac | |
| 10037B-A10 | VGB6 | C57BL/6NTac | |
| 10037B-B3 | VGB6 | C57BL/6NTac | |
| 10037B-C7 | VGB6 | C57BL/6NTac | |
| 10037B-C12 | VGB6 | C57BL/6NTac | |
| 10037B-F5 | VGB6 | C57BL/6NTac | |
