| Gene | Il1r2 |
| Chromosome | 1 |
| Deletion Start | 40,102,413 |
| Deletion End | 40,125,207 |
| Deletion Size | 22,795 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,125,049 | 40,125,068 | ACGTTCTCCTGGAGCATTGC |
| TUP | 40,125,071 | 40,125,100 | TGGCACCTCTGTCTCTGATCATCTTGGTTG |
| TUR | 40,125,107 | 40,125,126 | CCGTCTGCGCATCCATATTG |
| TDF | 40,125,049 | 40,125,068 | ACGTTCTCCTGGAGCATTGC |
| TDP | 40,125,071 | 40,125,100 | TGGCACCTCTGTCTCTGATCATCTTGGTTG |
| TDR | 40,125,107 | 40,125,126 | CCGTCTGCGCATCCATATTG |
PCR Primers
| Primer | Sequence |
| SU | TCAAACATTCTCGTTCTCAGG |
| SD | AAGGATGAGGAGGGACGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 284 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 407 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10038G-D3 | VGB6 | C57BL/6NTac | |
| 10038D-G2 | VGB6 | C57BL/6NTac | |
