| Gene | Epha1 |
| Chromosome | 6 |
| Deletion Start | 42,373,207 |
| Deletion End | 42,360,557 |
| Deletion Size | 12,651 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,372,848 | 42,372,869 | GCATTCCTACTGCAGCATCATG |
| TUP | 42,372,814 | 42,372,836 | ATGCCCACCCCTGACTTTGCAGC |
| TUR | 42,372,785 | 42,372,805 | AGGCTTTCCGCCTCAGACTTC |
| TDF | 42,361,117 | 42,361,135 | GATGGACAGCCCCAGAAGC |
| TDP | 42,361,090 | 42,361,113 | TGCCCATCGGATCTTCACCACAGC |
| TDR | 42,361,067 | 42,361,087 | TCCCAAAGCTCCACACATCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCTAGCAACACCCACTAG |
| SD | TGCTTCTTACCTTTCATCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10043A-D7 | VGB6 | C57BL/6NTac | |
| 10043A-E6 | VGB6 | C57BL/6NTac | |
| 10043A-F7 | VGB6 | C57BL/6NTac | |
| 10043B-G12 | VGB6 | C57BL/6NTac | |
| 10043B-G11 | VGB6 | C57BL/6NTac | |
