| Gene | Igfbp3 |
| Chromosome | 11 |
| Deletion Start | 7,213,787 |
| Deletion End | 7,208,365 |
| Deletion Size | 5,423 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,213,138 | 7,213,156 | CAGGACGAGCGGGTAGCAA |
| TUP | 7,213,105 | 7,213,130 | TGCCAGTGGACAGATCCCAGTCACTG |
| TUR | 7,213,084 | 7,213,103 | CCGCTCAGCGAGGAGACTCT |
| TDF | 7,208,458 | 7,208,478 | TTCTGCTGGTGTGTGGACAAG |
| TDP | 7,208,427 | 7,208,450 | AGCCCTTGCCAGGCTACGACACCA |
| TDR | 7,208,403 | 7,208,423 | GGCAATGTACGTCGTCTTTCC |
PCR Primers
| Primer | Sequence |
| SU | TCCGCCACCCCGGCTTCT |
| SD | ACTGACCCTGGTGTCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 298 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10044A-D7 | VGB6 | C57BL/6NTac | |
| 10044A-A1 | VGB6 | C57BL/6NTac | |
| 10044A-G10 | VGB6 | C57BL/6NTac | |
| 10044B-E6 | VGB6 | C57BL/6NTac | |
| 10044B-G4 | VGB6 | C57BL/6NTac | |
