| Gene | Ramp1 |
| Chromosome | 1 |
| Deletion Start | 91,196,793 |
| Deletion End | 91,223,361 |
| Deletion Size | 26,569 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,197,009 | 91,197,027 | ACAGGATGGAGCGGAAAGG |
| TUP | 91,197,044 | 91,197,072 | TCCTCCCACCCACATGTCATAAACATCTG |
| TUR | 91,197,078 | 91,197,096 | CTGGGCAGCCATCGAAGAC |
| TDF | 91,223,260 | 91,223,280 | AACAGCATCCTCTGCCCTTTC |
| TDP | 91,223,282 | 91,223,304 | TTGCGCTCCCCATTACGGTCACG |
| TDR | 91,223,310 | 91,223,330 | CAGACCACCAGTGCAGTCATG |
PCR Primers
| Primer | Sequence |
| SU | AACTCAGACAGGTGTTATCTC |
| SD | TCTTCCTTGGAGTTCAGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 427 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10048D-B7 | VGB6 | C57BL/6NTac | |
| 10048C-G8 | VGB6 | C57BL/6NTac | |
