| Gene | Unc93b1 |
| Chromosome | 19 |
| Deletion Start | 3,935,240 |
| Deletion End | 3,948,942 |
| Deletion Size | 13,703 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,935,669 | 3,935,687 | GGACCCTGGATGGGAAACC |
| TUP | 3,935,696 | 3,935,719 | CTCCAGACCCTATCCCGGCTTCCA |
| TUR | 3,935,740 | 3,935,759 | GCGGGCCAATCACTAATCAC |
| TDF | 3,948,692 | 3,948,712 | AGAAGTTGCAGCAAGGATTGG |
| TDP | 3,948,724 | 3,948,743 | CCGCGCATTCCGAAGCCACA |
| TDR | 3,948,745 | 3,948,763 | GGTAGCCGCGGACTTTGTG |
PCR Primers
| Primer | Sequence |
| SU | CACACCATGGACAGTATTAG |
| SD | GGGCAAGAAATGTGCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 252 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 395 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10049A-B3 | VGB6 | C57BL/6NTac | |
| 10049A-B7 | VGB6 | C57BL/6NTac | |
| 10049A-C5 | VGB6 | C57BL/6NTac | |
| 10049A-F5 | VGB6 | C57BL/6NTac | |
| 10049A-G9 | VGB6 | C57BL/6NTac | |
| 10049A-G10 | VGB6 | C57BL/6NTac | |
