| Gene | Htr3b |
| Chromosome | 9 |
| Deletion Start | 48,959,320 |
| Deletion End | 48,935,881 |
| Deletion Size | 23,440 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,959,288 | 48,959,309 | CACCAGGCAGCTCTTACAACAG |
| TUP | 48,959,239 | 48,959,262 | ACAACTGGGCTGAGGCCACCACTG |
| TUR | 48,959,208 | 48,959,228 | AATACAGCATGGACGCAAAGG |
| TDF | 48,936,023 | 48,936,043 | CGGGATCAGATTCACCAGAAG |
| TDP | 48,935,983 | 48,936,009 | TGGCCATCCTGTATCGCTTTGATCAGC |
| TDR | 48,935,955 | 48,935,974 | AGCACGGCAAGGTAGATTCG |
PCR Primers
| Primer | Sequence |
| SU | GCACTTGGGCCATCGGCTC |
| SD | TCCTTAAACCTAAGTGTACGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 436 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 359 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10050A-H4 | VGB6 | C57BL/6NTac | |
| 10050A-F4 | VGB6 | C57BL/6NTac | |
| 10050B-D9 | VGB6 | C57BL/6NTac | |
| 10050B-H7 | VGB6 | C57BL/6NTac | |
| 10050B-H5 | VGB6 | C57BL/6NTac | |
