| Gene | Stoml2 |
| Chromosome | 4 |
| Deletion Start | 43,031,370 |
| Deletion End | 43,028,144 |
| Deletion Size | 3,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,031,334 | 43,031,352 | GGCACTGGAGCCCTTTTGC |
| TUP | 43,031,292 | 43,031,315 | AACCTTCACCAGAGGCTCGGGACC |
| TUR | 43,031,257 | 43,031,277 | CCCACTCCTTCCCAGCTCAAG |
| TDF | 43,029,013 | 43,029,034 | TGAGCAGTATGTCAGCGCATTC |
| TDP | 43,028,986 | 43,029,011 | CCAAACTGGCCAAGGATTCCAACACA |
| TDR | 43,028,963 | 43,028,982 | CTGGGATTGGAGGGCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | GTGGGTACAGAATTTGGTGG |
| SD | GACTTCACTTCACATGAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 437 bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 367 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10051A-B7 | VGB6 | C57BL/6NTac | |
| 10051B-D1 | VGB6 | C57BL/6NTac | |
