| Gene | Tmem176b |
| Chromosome | 6 |
| Deletion Start | 48,838,354 |
| Deletion End | 48,834,028 |
| Deletion Size | 4,327 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,838,219 | 48,838,239 | TGGGCTCCCTGAAGAAGTTTC |
| TUP | 48,838,182 | 48,838,207 | CAGGCCAGAATCCACTATGGGCAGCT |
| TUR | 48,838,147 | 48,838,167 | TCTGTGCCACCTCAGGAACTC |
| TDF | 48,834,266 | 48,834,284 | CTCTGCCCCTCACCTTTCC |
| TDP | 48,834,226 | 48,834,250 | CCTTGCTGTCATTCCCTGTCGGTCC |
| TDR | 48,834,202 | 48,834,222 | CGCACTTGAAAGCAGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGGAGCACCGAACATTCAAACG |
| SD | GCCCACACCTTTCTGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10052A-C3 | VGB6 | C57BL/6NTac | |
| 10052A-E1 | VGB6 | C57BL/6NTac | |
| 10052A-E9 | VGB6 | C57BL/6NTac | |
| 10052A-A7 | VGB6 | C57BL/6NTac | |
| 10052A-B8 | VGB6 | C57BL/6NTac | |
