| Gene | Kcne3 |
| Chromosome | 7 |
| Deletion Start | 100,184,158 |
| Deletion End | 100,184,492 |
| Deletion Size | 335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,182,688 | 100,182,709 | GCTCTGGGCCTTTCCTGTTAAC |
| TUP | 100,182,720 | 100,182,743 | TGGCTGCTTCCAGATGCACCTTGC |
| TUR | 100,182,747 | 100,182,767 | TGTCCCACAACCCCTCAGTTC |
| TDF | 100,184,192 | 100,184,213 | CGGGACTGAGACCTGGTACATG |
| TDP | 100,184,216 | 100,184,240 | CCTCCATGCTGTGCTGAAGGCTCTG |
| TDR | 100,184,250 | 100,184,271 | GGCAGAGCAAGTGACTGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACGCACTGTGGGATGCTTC |
| SD | ATGTGTTCATGGGCTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 355 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10053F-G6 | VGB6 | C57BL/6NTac | |
| 10053F-H8 | VGB6 | C57BL/6NTac | |
| 10053F-G10 | VGB6 | C57BL/6NTac | |
| 10053G-C12 | VGB6 | C57BL/6NTac | |
| 10053G-F11 | VGB6 | C57BL/6NTac | |
