| Gene | Tmem144 |
| Chromosome | 3 |
| Deletion Start | 79,839,243 |
| Deletion End | 79,814,045 |
| Deletion Size | 25,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,838,664 | 79,838,686 | TGCGGCAGGACTGTCACCTAGGC |
| TUP | 79,838,639 | 79,838,658 | TGTGGAGAGGGATGCATGTG |
| TUR | 79,838,691 | 79,838,714 | GAACGCTGCTCTCAGTTAAAGAAA |
| TDF | 79,814,971 | 79,814,999 | TTTCTTTGAGACTCAGGCCATGACTGTGC |
| TDP | 79,814,949 | 79,814,967 | TGAATTGCCTCATCCCGAC |
| TDR | 79,815,003 | 79,815,023 | ATGTCTGCTTACAGCGGGAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGATTGATAGTACAAATG |
| SD | AAGTAGACATTGTTAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 51 bp | + | + | - |
| TDF | TDR | 53 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10057A-A10 | VGB6 | C57BL/6NTac | |
| 10057A-E7 | VGB6 | C57BL/6NTac | |
| 10057A-H6 | VGB6 | C57BL/6NTac | |
