| Gene | Adam33 |
| Chromosome | 2 |
| Deletion Start | 131,063,511 |
| Deletion End | 131,051,270 |
| Deletion Size | 12,242 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,062,893 | 131,062,912 | CGGGACGACAGGGCTCTTAG |
| TUP | 131,062,858 | 131,062,886 | CAAGCCTGTAGGGTGAATCTCCAAACTCC |
| TUR | 131,062,836 | 131,062,856 | GCCAGCCTAGTGCTTGCTATG |
| TDF | 131,051,850 | 131,051,868 | GGCAGAGCACGGCTAAACG |
| TDP | 131,051,815 | 131,051,840 | AAGGCACAGTTCCCACCAAGGGAGAC |
| TDR | 131,051,787 | 131,051,807 | GGCCCACCTAGAGCTGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGGTTCTCTAAGAACC |
| SD | AGAAGAACCACAGTGTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10062A-F10 | VGB6 | C57BL/6NTac | |
| 10062B-B10 | VGB6 | C57BL/6NTac | |
