| Gene | Taar1 |
| Chromosome | 10 |
| Deletion Start | 23,920,389 |
| Deletion End | 23,921,404 |
| Deletion Size | 1,016 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,920,595 | 23,920,615 | ATGGCCATTGTCGACTTTCTG |
| TUP | 23,920,617 | 23,920,641 | CTGCAGGGCATTATCAGACAGCCCA |
| TUR | 23,920,650 | 23,920,669 | CCAACAGCGCTCAACAGTTC |
| TDF | 23,921,132 | 23,921,151 | GAAACAAAAGCCGCGAAGAC |
| TDP | 23,921,153 | 23,921,178 | AGGAAAACGCCCACCATGATCCCTAA |
| TDR | 23,921,180 | 23,921,199 | AAGAACGGGCACCAGCATAC |
PCR Primers
| Primer | Sequence |
| SU | ACTCTTCACCAAGAATGTGG |
| SD | AAAAGATCATCTAAGGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 556 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10063E-C9 | VGB6 | C57BL/6NTac | |
| 10063E-C11 | VGB6 | C57BL/6NTac | |
| 10063E-E4 | VGB6 | C57BL/6NTac | |
| 10063E-F5 | VGB6 | C57BL/6NTac | |
| 10063E-H9 | VGB6 | C57BL/6NTac | |
