| Gene | Calcb |
| Chromosome | 7 |
| Deletion Start | 114,719,726 |
| Deletion End | 114,722,137 |
| Deletion Size | 2,412 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,720,148 | 114,720,170 | TGCAGGACTATGAGCAGATGAAG |
| TUP | 114,720,173 | 114,720,199 | TCTTGCTCCTCCTGTTCCAGCTTCCTG |
| TUR | 114,720,202 | 114,720,223 | GAGACTTACCGGGAGCCCTTAG |
| TDF | 114,721,317 | 114,721,337 | TCTGTTGCGTGTGCATTTCTC |
| TDP | 114,721,344 | 114,721,370 | CCGACACACTAAAGCTGCTGTTGCCAG |
| TDR | 114,721,372 | 114,721,391 | CCACTCTGGGCCACTGATTC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTTCACAGATGAGAG |
| SD | AGCTGGGCCCATGTGTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 384 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 396 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10064A-A10 | VGB6 | C57BL/6NTac | |
| 10064A-B12 | VGB6 | C57BL/6NTac | |
| 10064A-D11 | VGB6 | C57BL/6NTac | |
| 10064A-F2 | VGB6 | C57BL/6NTac | |
| 10064A-H3 | VGB6 | C57BL/6NTac | |
| 10064A-H7 | VGB6 | C57BL/6NTac | |
