| Gene | Lcn8 |
| Chromosome | 2 |
| Deletion Start | 25,653,125 |
| Deletion End | 25,656,115 |
| Deletion Size | 2,991 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,653,445 | 25,653,469 | GGATCTCTCCCCTCTCTGAGTTCTC |
| TUP | 25,653,476 | 25,653,501 | AGCAACTCCGCCTCAAGACAGGTGGA |
| TUR | 25,653,505 | 25,653,526 | CCCTTGAAGTGACCAGCTTGTG |
| TDF | 25,655,296 | 25,655,315 | CAGCTCGGAGCCTTGAGAAC |
| TDP | 25,655,326 | 25,655,347 | TCCCGGAACAGCCAGAAGCCTG |
| TDR | 25,655,349 | 25,655,368 | AGGCCTTGGTCTGCTGTCAT |
PCR Primers
| Primer | Sequence |
| SU | ATGGAGGCAGCTTAACTGTC |
| SD | AGAAAATGCAGGGCTGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 271 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10066E-C3 | VGB6 | C57BL/6NTac | |
| 10066C-B2 | VGB6 | C57BL/6NTac | |
