| Gene | Lrg1 |
| Chromosome | 17 |
| Deletion Start | 56,121,883 |
| Deletion End | 56,119,923 |
| Deletion Size | 1,961 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,120,684 | 56,120,702 | TGCACCTCTCGAGCAATCG |
| TUP | 56,120,660 | 56,120,681 | TGCAGGCGCTGTCTCCCGAGTT |
| TUR | 56,120,609 | 56,120,628 | AGAGCATTGCGGGTCAGATC |
| TDF | 56,120,095 | 56,120,115 | GGCTTCGACATCTCCCACAAC |
| TDP | 56,120,033 | 56,120,055 | TGGCTGGTCGCCAACCGAAACAA |
| TDR | 56,120,009 | 56,120,029 | CGCGTGTCGTTCTGTGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCTGGTGGGAGAGGACTC |
| SD | GACCCCTGAAACAGACGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10067A-A2 | VGB6 | C57BL/6NTac | |
| 10067A-A5 | VGB6 | C57BL/6NTac | |
| 10067A-C6 | VGB6 | C57BL/6NTac | |
| 10067A-F12 | VGB6 | C57BL/6NTac | |
| 10067A-G5 | VGB6 | C57BL/6NTac | |
| 10067A-G10 | VGB6 | C57BL/6NTac | |
| 10067C-D4 | VGB6 | C57BL/6NTac | |
| 10067C-E11 | VGB6 | C57BL/6NTac | |
| 10067C-G9 | VGB6 | C57BL/6NTac | |
