| Gene | Robo4 |
| Chromosome | 9 |
| Deletion Start | 37,402,039 |
| Deletion End | 37,413,559 |
| Deletion Size | 11,521 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,402,625 | 37,402,643 | CCCAGCCAAGATGAGGTGC |
| TUP | 37,402,649 | 37,402,672 | ATCCGGCCAACCACCTCCCACTAT |
| TUR | 37,402,676 | 37,402,694 | CTGCCCATTCAGCAGCCAG |
| TDF | 37,413,163 | 37,413,182 | CCTCCCTGGCCTCCTGATTC |
| TDP | 37,413,184 | 37,413,207 | CCAACTTCGCTGGGAAGAGGCCCT |
| TDR | 37,413,220 | 37,413,239 | TTACCACCAGCCTTGGGCAC |
PCR Primers
| Primer | Sequence |
| SU | AGCTGAAGTGTAGAGGTCAG |
| SD | TCCTTGCATGGTGTGACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 363 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10070A-B12 | VGB6 | C57BL/6NTac | |
| 10070A-D3 | VGB6 | C57BL/6NTac | |
| 10070A-F2 | VGB6 | C57BL/6NTac | |
| 10070A-E6 | VGB6 | C57BL/6NTac | |
| 10070A-A2 | VGB6 | C57BL/6NTac | |
