| Gene | Antxr1 |
| Chromosome | 6 |
| Deletion Start | 87,312,378 |
| Deletion End | 87,282,676 |
| Deletion Size | 29,703 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,312,332 | 87,312,352 | CGTGGAGCAGTTGGCTCATAG |
| TUP | 87,312,300 | 87,312,321 | AAGAGACCGCGGCGCACACTCA |
| TUR | 87,312,266 | 87,312,284 | GCAGGTGGGCTGGATTCAC |
| TDF | 87,283,223 | 87,283,242 | TGAGCCCAACAAAGCAATGG |
| TDP | 87,283,198 | 87,283,221 | CCTTTCACACCAGCCAAGGGCAGG |
| TDR | 87,283,167 | 87,283,186 | CTGTTGCGCCAGGTGATGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTGTCTGCTTCACTGTCAG |
| SD | AGACTCTGAGAACAGAAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 451 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10072D-B6 | VGB6 | C57BL/6NTac | |
| 10072H-B7 | VGB6 | C57BL/6NTac | |
| 10072H-D2 | VGB6 | C57BL/6NTac | |
| 10072H-D10 | VGB6 | C57BL/6NTac | |
| 10072H-G1 | VGB6 | C57BL/6NTac | |
| 10072I-F4 | VGB6 | C57BL/6NTac | |
| 10072I-E2 | VGB6 | C57BL/6NTac | |
