| Gene | Ms4a3 |
| Chromosome | 19 |
| Deletion Start | 11,639,293 |
| Deletion End | 11,629,638 |
| Deletion Size | 9,656 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,639,229 | 11,639,251 | CTGTTTATCAGCCCTTGGATGAG |
| TUP | 11,639,200 | 11,639,225 | CGCCATGTTCAACGAGGTGTACTGCA |
| TUR | 11,639,163 | 11,639,189 | CCAACTTTATAGCAAAGGCTGACTTAC |
| TDF | 11,629,854 | 11,629,877 | AAATCATCAGGGCAGAAATCTTTG |
| TDP | 11,629,827 | 11,629,844 | ACCACCATGATCACTATC |
| TDR | 11,629,801 | 11,629,823 | CCCTGTTCACTCCATCCTTTCTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGGATTTGCTTTGTGAAGAG |
| SD | AGAAAGGGGAACAAGCGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10073A-A9 | VGB6 | C57BL/6NTac | |
| 10073A-C9 | VGB6 | C57BL/6NTac | |
| 10073A-F3 | VGB6 | C57BL/6NTac | |
| 10073A-F12 | VGB6 | C57BL/6NTac | |
| 10073A-G5 | VGB6 | C57BL/6NTac | |
| 10073A-G11 | VGB6 | C57BL/6NTac | |
