| Gene | Fam180a |
| Chromosome | 6 |
| Deletion Start | 35,325,911 |
| Deletion End | 35,313,505 |
| Deletion Size | 12,407 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,325,653 | 35,325,673 | AGGAAATACCCGGTGGAAGAG |
| TUP | 35,325,622 | 35,325,644 | CCATCGGGCTCCCAAATTGGCTG |
| TUR | 35,325,600 | 35,325,619 | CAACCCGATGGCTTCTCAAC |
| TDF | 35,314,525 | 35,314,545 | TGAAAATGGGAGCCACAAACC |
| TDP | 35,314,491 | 35,314,519 | TCCACAGCACTGCTTTGTGTCTTTCTGAA |
| TDR | 35,314,465 | 35,314,485 | CCGCCTCCCTTTTCTGTTCTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGTTAGGTTAAGAGAG |
| SD | CTTAGAACCAAAGGAATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 441 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10077B-A8 | VGB6 | C57BL/6NTac | |
| 10077B-C10 | VGB6 | C57BL/6NTac | |
| 10077B-E5 | VGB6 | C57BL/6NTac | |
| 10077B-F4 | VGB6 | C57BL/6NTac | |
| 10077B-F11 | VGB6 | C57BL/6NTac | |
| 10077B-H9 | VGB6 | C57BL/6NTac | |
