| Gene | Sectm1a |
| Chromosome | 11 |
| Deletion Start | 121,075,653 |
| Deletion End | 121,068,703 |
| Deletion Size | 6,951 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,075,363 | 121,075,382 | AGCAATGCTCGGAGCTAACG |
| TUP | 121,075,320 | 121,075,346 | ACTGCCCAAAACTACACCCAGACAGCA |
| TUR | 121,075,299 | 121,075,318 | GCAAAGGCACCCTCTTTTCC |
| TDF | 121,068,860 | 121,068,880 | AACCCCACCCCTGACTGTTAC |
| TDP | 121,068,831 | 121,068,856 | CCCACTTCAGATCACGCGAAGAGCTC |
| TDR | 121,068,810 | 121,068,829 | CCAGGCTTGCCCTCTATTGG |
PCR Primers
| Primer | Sequence |
| SU | AAAGCTCTATAGGAGAATTG |
| SD | TAGGATGGACTGACTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10078B-A8 | VGB6 | C57BL/6NTac | |
| 10078B-B8 | VGB6 | C57BL/6NTac | |
| 10078B-C4 | VGB6 | C57BL/6NTac | |
| 10078B-C11 | VGB6 | C57BL/6NTac | |
| 10078B-D2 | VGB6 | C57BL/6NTac | |
| 10078B-G11 | VGB6 | C57BL/6NTac | |
