10079   Metrnl     Status: ES cell colonies screened / QC positives

GeneMetrnl
Chromosome11
Deletion Start121,702,611
Deletion End121,716,525
Deletion Size13,915
Genome Build38
MGI IDMGI:2384806
NCBI Gene ID210029
BAC ID475g24
BAC LibraryBAC ES release 2
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,702,912121,702,931CGCCCTAGCTCTGGTTTCAG
TUP121,702,937121,702,961TGGGACCCAGATGCCACCAACTCTT
TUR121,702,963121,702,982GAGAGTGGGCGTCTTCCGAG
TDF121,716,162121,716,181AGCCTGGTGCTAGAACTGGG
TDP121,716,190121,716,214TGCTGCTCTGACCCCTTAGGTCCCA
TDR121,716,217121,716,235ATGGGTCAGGGCATCCTTG

PCR Primers

PrimerSequence
SUGAACTTGCCAGCTCAGAGG
SDGTTCAGTGGGACAGATGCAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev330 bp-++
TUFTUR71 bp++-
TDFTDR74 bp++-
NeoFwdSD303 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10079C-A5VGB6C57BL/6NTac
10079C-H9VGB6C57BL/6NTac
10079C-G10VGB6C57BL/6NTac
10079C-F8VGB6C57BL/6NTac
10079C-D10VGB6C57BL/6NTac
10079C-C5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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