| Gene | Gprc6a |
| Chromosome | 10 |
| Deletion Start | 51,631,428 |
| Deletion End | 51,614,833 |
| Deletion Size | 16,596 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,631,363 | 51,631,387 | TGATACCTCCCAGTCTTGTCATACC |
| TUP | 51,631,328 | 51,631,357 | TGACTTCGTGGCTATCACTTCTCCTGGACA |
| TUR | 51,631,304 | 51,631,324 | GCAAACAAGCCACCAATCATG |
| TDF | 51,615,046 | 51,615,064 | TGGACAGCCTTGCCTTGAG |
| TDP | 51,615,006 | 51,615,034 | TGGACTCCACCAGCTACGATACTGCAACA |
| TDR | 51,614,984 | 51,615,004 | TGTTACCAGGGCTCTGATTGG |
PCR Primers
| Primer | Sequence |
| SU | ATCAGTTCATCTCCAGCTC |
| SD | AGATGAAAATCTGAGATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 398 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10080A-D3 | VGB6 | C57BL/6NTac | |
| 10080B-F8 | VGB6 | C57BL/6NTac | |
