| Gene | Il1f10 |
| Chromosome | 2 |
| Deletion Start | 24,291,208 |
| Deletion End | 24,293,807 |
| Deletion Size | 2,600 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,292,774 | 24,292,792 | TACACACGGAATGGCCAGC |
| TUP | 24,292,794 | 24,292,819 | CCTGCTGGGAGACCCTGATTCAGACA |
| TUR | 24,292,826 | 24,292,845 | CACCGGAAGATCACCTGGAC |
| TDF | 24,293,176 | 24,293,196 | TCCTTCCTAACCGAGGCCTAG |
| TDP | 24,293,198 | 24,293,222 | CCGCTCCAAGGTCCCCATCTTCCTG |
| TDR | 24,293,228 | 24,293,246 | GCAGCAACTTCCTCCCTGC |
PCR Primers
| Primer | Sequence |
| SU | TGCAATGGAGTACCACTCAG |
| SD | GAGAGCTCTCATCATTTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 396 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 373 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10081B-F9 | VGB6 | C57BL/6NTac | |
| 10081B-C4 | VGB6 | C57BL/6NTac | |
