| Gene | Cd300e |
| Chromosome | 11 |
| Deletion Start | 115,062,021 |
| Deletion End | 115,053,240 |
| Deletion Size | 8,782 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,061,806 | 115,061,830 | CAGTGTAGTCCAGCATCCATCTATG |
| TUP | 115,061,782 | 115,061,802 | CCTACAAACCATTACTAAGCA |
| TUR | 115,061,760 | 115,061,780 | AACAGTCCAGGGTTGCAGTTG |
| TDF | 115,053,404 | 115,053,426 | TGGAAAGAGACAGGTTGGTTCTG |
| TDP | 115,053,360 | 115,053,386 | TATCTTCCATCTTCATTTGCCCGCAGG |
| TDR | 115,053,320 | 115,053,341 | GGAAGACCAGGACCTGGAACTG |
PCR Primers
| Primer | Sequence |
| SU | TCAGGCTGTGACAAACGTGC |
| SD | TGCCTTTACTCCCAGTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10083A-H1 | VGB6 | C57BL/6NTac | |
| 10083B-A7 | VGB6 | C57BL/6NTac | |
