| Gene | Sema3g |
| Chromosome | 14 |
| Deletion Start | 31,217,914 |
| Deletion End | 31,228,206 |
| Deletion Size | 10,293 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,218,009 | 31,218,031 | GCGGCTCTCCTACAGAGGTACTG |
| TUP | 31,218,037 | 31,218,061 | CAGCATGCACACAAACAGGACAGCA |
| TUR | 31,218,073 | 31,218,094 | TGTCCTAGAGCCACGGACATTC |
| TDF | 31,227,868 | 31,227,886 | TTTGCCCTGGAGGTGATTG |
| TDP | 31,227,893 | 31,227,917 | TGCAACTGGACAGCCTGTTCCTTCG |
| TDR | 31,227,921 | 31,227,941 | GAGGGCTCCTCTAGCCTTGAC |
PCR Primers
| Primer | Sequence |
| SU | TAGCTGCACGGGCATTGAGC |
| SD | ACCACCCGCCTTGCCTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 403 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10084B-C2 | VGB6 | C57BL/6NTac | |
| 10084B-D12 | VGB6 | C57BL/6NTac | |
| 10084B-G3 | VGB6 | C57BL/6NTac | |
