| Gene | Lrrc14 |
| Chromosome | 15 |
| Deletion Start | 76,712,876 |
| Deletion End | 76,714,749 |
| Deletion Size | 1,874 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,713,555 | 76,713,573 | CCAGTCCCCGTGGAAATCC |
| TUP | 76,713,580 | 76,713,604 | ACCTTCGGGTAAACCGGGCCTCGTA |
| TUR | 76,713,607 | 76,713,625 | AGGGCCTCACGAAGGAATG |
| TDF | 76,714,540 | 76,714,560 | CGGGTGGAAGCTGAGTTACAC |
| TDP | 76,714,582 | 76,714,603 | CGTGCCCATGTGCTCTGGACCA |
| TDR | 76,714,606 | 76,714,625 | GCTAGCCGGCCATAGATGTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCCAGACCTAGAAACTTATC |
| SD | GCCCATAAATTCACATTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 299 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 379 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10085B-A7 | VGB6 | C57BL/6NTac | |
| 10085B-B1 | VGB6 | C57BL/6NTac | |
| 10085B-C7 | VGB6 | C57BL/6NTac | |
| 10085B-D1 | VGB6 | C57BL/6NTac | |
| 10085B-F2 | VGB6 | C57BL/6NTac | |
| 10085B-H5 | VGB6 | C57BL/6NTac | |
