| Gene | Ucn3 |
| Chromosome | 13 |
| Deletion Start | 3,941,647 |
| Deletion End | 3,941,156 |
| Deletion Size | 510 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,941,329 | 3,941,348 | ATCCCAGGCACAGCACAAGG |
| TUP | 3,941,297 | 3,941,322 | TGTACCCAGACAAGCCCAAAAGCGAC |
| TUR | 3,941,277 | 3,941,295 | AGGGTGAACTTGGTGCCCC |
| TDF | 3,941,353 | 3,941,371 | GGGAGCACCCGGTACAGAT |
| TDP | 3,941,329 | 3,941,351 | CCAATCCCAGGCACAGCACAAGG |
| TDR | 3,941,296 | 3,941,314 | GGTCGCTTTTGGGCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCCAAGCCACCTCTTCTGG |
| SD | GGGGAGAGACGTTAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 286 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10092A-C4 | VGB6 | C57BL/6NTac | |
| 10092A-C8 | VGB6 | C57BL/6NTac | |
| 10092A-E5 | VGB6 | C57BL/6NTac | |
| 10092A-F4 | VGB6 | C57BL/6NTac | |
